If something is rotting in your kitchen right now,use it and make out of it life saving antibiotics! Penicillin has been a lot longer than folks think. Actually the American Indians used it centuries before the white man ever ventured onto America’s shores. The blue and white penicillin molds makes penicillin. Making penicillin at home is difficult, but possible in case you really have the correct gear and ingredients.
You may need:
- A g scale
- Separator funnel
- A 1-liter glass container
- 750 ml Erlenmeyer flask with a non absorbent plug
- A pH test kit
- 2 pieces of whole wheat bread
- A cantaloupe rind, more bread, or citrus fruit
– let it mold and Set out the rind, bread or fruit. It’s going to go through several periods. The mold will be gray or white, then it’ll turn blue, then a bright blue green. This is actually the colour you would like. Note: if you choose to utilize bread, it’s finest to make it yourself because many bakeries use an ingredient that inhibits mold growth.
–Sterilize the flask by putting it in the pressure cooker at 15 lb. for at least 15 minutes, or bake it at 315 degrees F for an hour.
–Cut the whole wheat bread (see note in step 1) into 1/2-inch cubes and place them in the flask, attentive to be as sterile as you can.
–Scrape the blue green mold from the host and put it in with the bread. Again, be as clean with this particular measure as you can, for example, boil the tongs that you’re using.
– allow it to incubate for 5 days and Set the flask in a dim place that’s around 70 degrees.
–Now it ’s going to get complicated. You’re going to need these ingredients:
- Lactose Monohydrate 44 gm
- Corn Starch 25 gm
- Sodium Nitrate 3 gm
- Magnesium Sulfate 0.25 gm
- Potassium Monophosphate 0.50 gm
- Glucose Monohydrate 2.75 gm
- Zinc Sulfate 0.044 gm
- Manganese Sulfate 0.044 gm
–Sterilize the 1 liter container dissolve the above ingredients in 500 ml of cold water. Add more cold water to make it a complete liter.
–Use hydrochloric acid (HCL) to adjust the pH to 5.0-5.5 using your pH test kit.
–Sterilize the container together with the solution as described above.
–Allow the solution to cool, then add the mold. Incubate it for another 7 days under the same conditions as before.
It ’s time to pull the penicillin that ’s infused in the fluid.
– Filter the mixture through a coffee filter or cheesecloth that is sterilized.
–Adjust the pH of the solution to 2.2 using the HCL and the pH test kit.
–Mix with cold ethyl acetate in the separatory funnel and shake for 30 seconds or so then allow it to separate. The ethyl acetate will probably be at the base.
A beaker in an ice bath Chills and drain the ethyl acetate into it. Add 1 percent potassium acetate and mix it again.
–Let while the solution remains in the beaker, the ethyl acetate evaporate away. You want lots of air.
–You have penicillin, assuming you did everything right. Actually the crystals that stay are potassium penicillin and potassium acetate.
Safest way for using it is by building a poultice or patch and applying it to the afflicted area. But if the infection is internal, you’ll need to dry the material up, grind it to a powder, then feed it to whoever is sick.
Penicillin works against gram-positive bacteria, like Staphylococcus and Pneumococcus by interrupting bacterial cell wall synthesis causing the cells to take on extra water, which causes them to explode. Effectiveness against gram-negative bacteria (such as E. coli and K. pneumonia) is limited, with quite high concentrations of penicillin needed to kill those organisms, which have membranes that protect them from penicillin.
Some gram-positive bacteria have developed the power to survive penicillin vulnerability (become resistant to it) by generating penicillinases, enzymes that degrade penicillin.
Penicillins paved the way for some other antibiotics, for example cephalosporins, streptomycin from Actinomycetes Streptomyces, and made from the fungus Acremonium.